The process of In Vitro Fertilization
1.-A previous study of the couple
A complete study of the couple is needed to decide what type of technique is needed (IVF or ICSI), especially the malefactor. In some cases it is necessary to resort to donated gametes and, therefore, a selection of donors must be made. The use of one technique or another will depend on the etiology of the member of the couple that will use their own gametes.
2.-Controlled ovarian stimulation
The woman must be medicated in order to obtain a greater number of oocytes in that cycle. Ovarian stimulation is controlled by vaginal ultrasound to follow the growth of the ovaries follicles and analytical to know the hormonal levels for, based on these two parameters, go regulating the dose of medication.
3.-Obtaining oocytes and processing gametes
To obtain the oocytes from the inside of the follicles, a vaginal aspiration-aspiration is performed with ultrasound control. This intervention lasts between ten and fifteen minutes and is done under sedation. Once evaluated in the laboratory and known the degree of maturity, the sample of semen is requested to the couple for its preparation in the laboratory. Both the ovules and the sperm are processed to optimize their fertilizing capacity.
The endometrium of the recipient is synchronized with the stage of development of the embryos, and the embryo transfer is carried out at the appropriate time (between days 3 and 6 of embryo culture).
For the endometrium to transform and go from being proliferative (while developing in the first phase of the cycle) to secretory endometrium (which is how it should be to receive the embryos and that they can implant), what is done is to add progesterone to the estrogen that the recipient carries in her THS cycle. This therapy is maintained until the pregnancy test, which, if positive, will last until week 10-12 of the same.
5.- Vitrification of remaining embryos
If at the time of the embryo transfer there are viable surplus embryos, that is, of good quality, they will be vitrified and kept frozen for later use by the couple. If pregnancy is achieved and vitrified embryos remain, you may be able to give a brother with the same parents.
✓ Pre-treatment visit / Cycle programming
✓ Psychological visit
✓ Pre-anesthetic visit
✓ Ovarian stimulation of patient and controls
✓ Ovarian puncture
✓ Processes and laboratory techniques
✓ Culture and embryo selection with Time-Lapse
✓ Embryo transfer
✓ Pregnancy test and first ultrasound
✘ Donor’s semen, if necessary.
✘ Cultivation to blastocyst
✘ Pre-implantation tests or genetic studies.